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In vitro expression of the RARs. Expression of the three RAR isotypes was evaluated in the motor neuron-enriched cell culture system. Polymerase chain reaction (PCR) analysis of RNA isolated from primary motor neuron cultures after 24 hours in culture. RARα and RARβ but not RARγ were expressed. “MN” denotes RNA from primary motor neurons; “+” denotes RNA isolated from JM2 cells used as a positive control. Cyclophilin was used as a PCR positive control for each cell type.
