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Illustration of evaluationmethod. For examinationof potential protective effectsby perineuronal nets the largestcross-sectional area wasdefined as reference area andcopied to the hemisphere ofinjection as well as its contralateralside. Within this area,counting of Fluoro-Jade B positiveneurons was performedwithin three rectangles of asize of 1.8mm2, randomlyplaced to the reference area.Scale bar 1000μm.
