Figure 4.

Quantification of differences by using HAG. Detecting differential effects on neuronal degeneration for neurons with and without PN with triple staining of degenerating net-associated neurons with immunocytochemical labeling of Fluoro-Jade B (a), NeuN (b) and HAG (c). Overlay image (d) was used for counting procedures. Net-associated neurons are significantly less severely affected by degeneration after FeCl₃ injection. This effect could be seen after 24h (e) as well as 72h (f).Statistical analyzis by Wilcoxon Range test, Scale bar 20μm.