Figure 4.

Therapeutic treatment with ER-886046 or standard of care drugs. Arthritis was induced in mice and allowed to develop to near its clinical peak as determined by visual clinical scoring (A). When SPECT detectable changes were noted (day 11), mice were stratified into groups and treatment with ER-886046, celecoxib or prednisolone was begun (A). X-rays were collected on paws from mice at the termination of the study (B) and the X-rays were scored for bone destruction. After being X-rayed paws were fixed in formalin and then processed for IHC analysis of F4/80 (C) and F4/80 staining was scored visually for intensity. Longitudinal in vivo imaging was performed throughout the study. Hind paws were imaged for bioluminescence using luminol for detection of MPO activity (D) and bone turnover in paws was detected using ^99mTc-MDP-SPECT imaging (E). Shown are means ± SEM results from 2 experiments with 14 animals per group. *p<0.05 and **p<0.01 significantly different from vehicle treatment.