Table 2.
RTPCR Confirmation of Affymetrix Gene Expression Fold Change. Above are shown the 6 genes that were selected for RT-PCR confirmation: mean fold change compares the average BCLP expression levels to those of the SCLP condition, in both the microarray and the RT-PCR
| Mean Fold Change | ||||
|---|---|---|---|---|
|
| ||||
| Time | Gene Symbol | Gene Name | Affymetrix | RTPCR |
| 2h post CLP | Slc1a4 | Solute carrier family 1 (glutamate/neutral amino acid transporter), member 4 | 2.69 | 3.47 |
| Angptl4 | Angiopoietin-like 4 | 0.33 | 0.36 | |
|
| ||||
| 4h post CLP | Pcolce | Procollagen C-endopeptidase enhancer | 3.05 | 6.43 |
| Nfkbia | Nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor, alpha | 0.49 | 0.77 | |
|
| ||||
| Day 7 post CLP | Stam2 | Signal transducing adaptor molecule (SH3 domain and ITAM motif) 2 | 2.49 | 1.07 |
| G6pd | Glucose-6-phosphate dehydrogenase | 0.42 | 0.54 | |