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. 2004 Mar;72(3):1318–1325. doi: 10.1128/IAI.72.3.1318-1325.2004

FIG. 4.

FIG. 4.

Flow cytometric analysis of CD25 expression on the cell surface of CHO transfectants stimulated with BfLP. CHO/CD14/TLR2, CHO/CD14/TLR4, and CHO/CD14 reporter cell lines were stimulated with BfLP for 15 h. Each of the reporter line contains a stably transfected ELAM-CD25 reporter gene and expresses human CD25 on its surface as a consequence of NF-κB activation. Stimulated cells were stained with a phycoerythrin-labeled anti-CD25 MAb and subjected to flow cytometry analysis to measure the expression of CD25. Cells stimulated with 10 mM OG/PBS were used as controls.