Figure 3.

Quantitative (ELISA) measurement of IRS-1 integrity and sub-cellular distribution. IRS-1 was captured with anti-C-terminal mAb, and detected with anti-ubiquitin, anti-N-terminal, and anti-C-terminal rabbit polyclonal antibodies. For total intact and ubiquitinated IRS-1, skeletal muscle tissues were homogenized with Cell Signaling buffer and subcellular components were isolated according to the Qiagen protocol. Values represent mean ± sem for 15 burned and 8 sham treated mice. *: p<0.05, **: p<0.005.