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. 2004 Mar;72(3):1755–1759. doi: 10.1128/IAI.72.3.1755-1759.2004

FIG. 4.

FIG. 4.

OspB F(ab)2 fragments interfered with the attachment and colonization of B. burgdorferi within I. scapularis. The distribution of B. burgdorferi within the I. scapularis gut 24 and 72 h after feeding is shown. Nymphal ticks fed on B. burgdorferi-infected mice that had been treated with either normal rabbit serum (NRS), F(ab)2 fragments from normal rabbit serum (N-Fab), or OspB MAb B22 or B27 or F(ab)2 fragments prepared from polyclonal anti-OspB sera (B-Fab). The spirochetes (arrows) were stained with a FITC-labeled goat anti-B. burgdorferi antibody (green), and the nuclei of the gut epithelial cells were stained with propidium iodide (red). Images were recorded at ×400 magnification and are presented as a merged image for clarity.