FIG 5 .

Phenazine-modulated inhibition of C. albicans wrinkled colony morphology is not solely due to decreased extracellular pH. (A and B) C. albicans colonies grown on Glu-AA-GlcNAc with the pH adjusted to 7.0 or 5.0 prior to inoculation. A total of 5 µM of PMS, PYO, or methylene blue (MB) was added to the medium when specified, and the pH indicator bromocresol purple, which yields a red-purple color under alkaline conditions and a yellow-orange color under acidic conditions, was included. In panel A, the medium was buffered with 40 mM MOPS (pH 7) or 100 mM citrate (pH 5) as indicated. Colonies were incubated for 48 h at 37°C.