FIG 6 .

Effects of phenazines on respiratory metabolism and oxygen consumption. (A) C. albicans colonies grown in Glu-AA medium alone or with 5 µM PMS or 20 µM PYO under noninducing conditions. The respiratory activity of colonies was assessed using TTC, and images were captured using a stereoscope at 10× (top). Internal oxygen measurements in similarly grown colonies were made from the top to the bottom of control colonies (black), with PMS (light gray) or PYO (dark gray). (B) Oxygen gradients in colonies grown under noninducing (smooth) and inducing conditions (wrinkled) were measured at room temperature. The respiratory activity in wrinkled colonies was greater in wrinkles (gray arrow) than in valleys (black arrow) (inset).