TABLE 2.
Oligonucleotides used in this study
| Primer name | Oligonucleotide sequencea | Target |
|---|---|---|
| HA-encoding primers | ||
| oVCU102 | GTC CGG GAC GTC GTA CGG GTA CGT CCT CGT CCC GCC | Plug |
| oVCU103 | CCG TAC GAC GTC CCG GAC TAC GCC GCG GGC AGC AGC GGC | Plug |
| oVCU112 | GTC CGG GAC GTC GTA CGG GTA GCT GCC GTC ATC AAC CGG | L2 |
| oVCU113 | CCG TAC GAC GTC CCG GAC TAC GCC AAG TAC GCC TAT TTC ATC GTT | L2 |
| oVCU114 | GTC CGG GAC GTC GTA CGG GTA GCC GTA TTT GTG GTT GCC | L3 |
| oVCU115 | CCG TAC GAC GTC CCG GAC TAC GCC GGA CTG TTT ACC AGC GGC | L3 |
| oVCU106 | GTC CGG GAC GTC GTA CGG GTA GTT GTT TTG AGG GGG CGT | L5 |
| oVCU107 | CCG TAC GAC GTC CCG GAC TAC GCC GGC AAA AAA ACC AGC CCC | L5 |
| oVCU116 | GTC CGG GAC GTC GTA CGG GTA TAT TTT ATC GCC CGA CCG C | L7 |
| oVCU117 | CCG TAC GAC GTC CCG GAC TAC GCC AAA GCC GTC AAA ATC GAT CC | L7 |
| oVCU128 | GTC CGG GAC GTC GTA CGG GTA GTC TGC GCG TTT TTT GAT GTC | L9 |
| oVCU129 | CCG TAC GAC GTC CCG GAC TAC GCC CGC ACC GAT ATT CAA TCA CAC | L9 |
| oVCU130 | GTC CGG GAC GTC GTA CGG GTA GCG GCT GTT GCC GTT GAG | L10 |
| oVCU131 | CCG TAC GAC GTC CCG GAC TAC GCC AAT ACA AAA GCC ACC GCG C | L10 |
| oVCU132 | GTC CGG GAC GTC GTA CGG GTA GTA AAC GCC GAC ATT TTT GTG | L11 |
| oVCU133 | CCG TAC GAC GTC CCG GAC TAC GCC AAC CGA TAT GCCb CCC GGC C | L11 |
| oVCU120 | GTC CGG GAC GTC GTA CGG GTA AAA ACG AAA ACC CGG GCG G | T2 |
| oVCU121 | CCG TAC GAC GTC CCG GAC TAC GCC GAA AAC AAA CGG CAC TAC ATC | T2 |
| oVCU122 | GTC CGG GAC GTC GTA CGG GTA GGC GGT ATC GAA GGA TTT TTT | T4 |
| oVCU123 | CCG TAC GAC GTC CCG GAC TAC GCC AAA ATC CGC CAC AAC CTG AG | T4 |
| oVCU134 | GTC CGG GAC GTC GTA CGG GTA GTA ACC GAG ATT CAC GCT CA | β9 |
| oVCU135 | CCG TAC GAC GTC CCG GAC TAC GCC GAC CGC TTC GGC TCT AAT C | β9 |
| oVCU124 | GTC CGG GAC GTC GTA CGG GTA ATT GAT GCC GGT AAT CCG C | β16 |
| oVCU125 | CCG TAC GAC GTC CCG GAC TAC GCC ATT TTG GGC AAA ATC GAT TGG | β16 |
| tbpA-specific primers | ||
| Tfbp2 | AGC GGC GCA TTG GCG GGC TC | |
| Tfbp3 | GGG GCG CAT CGG CGG TGC GG | |
| Tfbp5 | AAA ACA GTT GGA TAC CAT AC | |
| Tfbp13 | GGT CGT GGC TGT TCC GCC CG | |
| Tfbp15 | TAT AAG TAT TGT TGC CAA GA | |
| Tfbp17 | AAC CGA GAT TCA CGC TCA GG | |
| Tfbp18 | GCC ATA AGC TCT TGC AGG CG | |
| Tfbp22 | ATC GGC ATT GGT ATA GAC AT | |
| Tfbp24 | CCG CAC CCT GTC CTG GAA CG | |
| Tfbp28 | AAA TCC AGC CAG TCG GCA GG | |
| Tfbp32 | AAG CTC CGG CTA CTC GAT AC | |
| Tfbp36 | GCT TCC TCG CCG ATC CGC | |
| oVCU7 | CTC GAG GCT CTA GAA ACC CCA ACG CAG | |
| oVCU100 | ATG CAA CAG CAA CAT TTG TTC | |
| oVCU101 | GAA ACG GTG GGG ATT GTG |
a
Underlined sequence indicates the nucleotides that encode the HA epitope.
b
Mutagenic primer encoding alanine 847 deletion.