TABLE 3.
Affinity and capacity measurements generated from equilibrium-phase binding assays conducted with epitope insertion mutants
| Strain | Description of mutation | Kda (nM) | Capacitya (no. of receptors/ μg of TCPb) |
|---|---|---|---|
| FA6905 | Wild type | 10.4 ± 2.0 | 2.1 × 108 |
| MCV508 | PHA TbpA | 120.5 ± 74.8 | 3.3 × 108 |
| MCV528 | L2HA TbpA | 18.0 ± 5.3 | 1.8 × 108 |
| MCV512 | L3HA TbpA | ||
| MCV506 | L5HA TbpA | 14.8 ± 5.2 | 2.2 × 108 |
| MCV510 | L7HA TbpA | 8.7 ± 1.9 | 1.2 × 108 |
| MCV516 | L9HA TbpA | 20.6 ± 2.6 | 1.6 × 108 |
| MCV518 | L10HA TbpA | 5.8 ± 0.5 | 1.8 × 108 |
| MCV520 | L11HA TbpA | 28.6 ± 8.2 | 1.3 × 108 |
| MCV514 | T2HA TbpA | 6.6 ± 1.5 | 1.4 × 108 |
| MCV526 | T4HA TbpA | 21.3 ± 5.0 | 2.0 × 108 |
| MCV522 | β9HA TbpA | ||
| MCV524 | β16HA TbpA | 16.2 ± 6.6 | 7.8 × 107 |
a
Kd, capacity and error calculated with Grafit software.
b
TCP, total cellular protein determined by bicinchoninic acid assay.