FIG. 2.

Kinetics of the IFN-γ response in C57BL/6 mice immunized with subunit vaccines. Mice were immunized with either BCG or ESAT in a variety of adjuvants, and IFN-γ from the restimulation of PBMCs (in nanograms per milliliter) was measured as an indicator of systemic immune responses. Mice (four animals per group) were used for tissue samples either the day before the first vaccination (day −1), 1 week after the final vaccination (day 7), 3 weeks after the final vaccination (day 21), or 8 weeks after the final vaccination (day 56). Cells from blood or spleen were pooled within groups and restimulated in triplicate wells. Kinetic studies were performed with spleen and blood cells for all adjuvants assessed, using at least two time points after vaccination, with comparable results.