TABLE 1.

Protection against vertical transmission of N. caninum in pups born to BALB/c mice immunized with plasmid DNA encoding NcGRA7 antigen or with nonrecombinant plasmid DNA with or without CpG adjuvant^a

Vaccination	NcTZ vaccine challengeb	No. of positive pups/total			Vaccination	NcTZ vaccine challengeb	No. of positive pups/total
		Vaccine trial 1	Vaccine trial 2				Vaccine trial 1	Vaccine trial 2
pCMV-NcGRA7 + CpG	Yes	0/5	0/5
		0/8	2/5
		0/3	0/5
		1/7	0/1
		0/5	1/4
		3/8	1/5
		0/5
		4/8
		1/5
pCMV-NcGRA7	Yes	5/6	1/2
		3/5	0/5
		0/6	3/5
		0/4	3/5
		5/10	0/6
		1/6	0/5
		0/5	0/5
		3/6	2/3
		4/6	0/4
			0/2
pCMV-NRd + CpG	Yes	8/8	3/4
		9/9	5/6
		8/8	5/5
		5/7	4/4
		3/3	4/4
		6/8
		5/5
		8/8
		7/7
pCMV-NR	Yes	4/4	6/6
		5/5	4/4
		6/6	5/5
		6/6	5/6
		4/6	2/7
		6/6	6/8
		3/3	8/8
		5/6	3/3
		3/3	6/6
			9/9
NcTZc	Yes	0/1	0/7
		1/10	0/5
			0/6
			0/4
			0/6
PBS	Yes	8/8	6/6
		6/8	5/5
		5/6	7/7
		6/6	2/2
		9/9
		4/6
PBS	No	0/5	0/7
		0/7	0/4
		0/5	0/6
			0/3

^aData are expressed as the number of pups positive for N. caninum DNA b, PCR/total number of pups in the litter.

^bN. caninum tachyzoite challenge infection.

^cPBS-insoluble whole N. caninum tachyzoite antigen.

^dNR, nonrecombinant plasmid.