TABLE 5.
1H NMR chemical shifts for desferri form of fraction 4 at 293 K in DMSO-d6 confirm its identity as desferriferricrocin
| Structural group | Signal(s) at the following residuea:
|
|||||
|---|---|---|---|---|---|---|
| Glycine (1) | Serine (2) | Glycine (3) |
N5-Acetyl-N5-hydroxyl-l-ornithine
|
|||
| 4 | 5 | 6 | ||||
| NH | 8.54 | 8.08 | 8.35 | 8.08 | 7.91 | 7.84 |
| αCH | 3.90, 3.41 | 4.19 | 3.77, 3.69 | 3.97 | 4.17 | 4.00 |
| βCH | 3.66, 3.55 | 1.69, 1.56 | 1.67, 1.57 | 1.62, 1.49 | ||
| δCH | 1.50, 1.50 | 1.52, 1.52 | 1.53, 1.43 | |||
| γCH | 3.45, 3.45 | 3.41, 3.49 | 3.44, 3.44 | |||
| CH3 | 1.96 | 1.96 | 1.93 | |||
| OH | 3.50 | 9.72b | ||||
a
Numbers refer to the order in which residues occur in the ferricrocin molecule.
b
Broad NMR signal centered at 9.72 ppm and compatible with ornithine OH.