TABLE 5.

Leukocyte counts in BALF after supplementation with DHA

Mouse strain	No. of samples	Supplement	Relative no. (%) of indicated type of cell in BALF			Absolute no. of indicated type of cell in BALF (thousand cells/ml)
			AMd	PMNe	Lymphf	Leukocytes [ln(Leuk)]a	AMd [ln(AM)]	PMNe [ln(PMN)]	Lymphf [ln(Lymph)]
Wild typeg	8	Sham	74.8 ± 14.8	14.3 ± 6.0	10.9 ± 10.7	40.0 ± 34.9 [3.3 ± 1.0]	32.4 ± 31.3 [3.0 ± 1.1]	5.4 ± 4.8 [1.3 ± 1.1]	2.2 ± 1.4 [0.5 ± 0.9]
	6	DHA	69.2 ± 20.0	28.6 ± 20.9	2.2 ± 2.6	55.8 ± 20.0 [4.0 ± 0.4]	37.7 ± 16.9 [3.6 ± 0.4]	16.7 ± 13.9 [2.5 ± 1.0]	1.5 ± 1.9 [−0.3 ± 1.3]
Cftr knockout	6	Sham	66.9 ± 15.7	30.1 ± 16.3	3.1 ± 2.5	61.3 ± 37.4 [4.0 ± 0.6]	39.5 ± 19.0 [3.5 ± 0.6]	20.2 ± 20.4 [2.6 ± 1.0]	1.7 ± 1.3 [−0.7 ± 3.1]
	6	DHA	66.6 ± 29.9	32.5 ± 30.1	0.9 ± 1.0b	102.8 ± 57.5 [4.5 ± 0.6]b	59.1 ± 35.1 [4.0 ± 0.5]	42.7 ± 60.0 [2.9 ± 1.6]	1.1 ± 1.2 [−2.3 ± 3.8]

^aSignificantly different (one-way ANOVA or Kruskal-Wallis test; P ≤ 0.05).

^bSignificantly different from wild-type, sham-treated mice (Bonferroni's multiple comparisons; P ≤ 0.05).

^cAll mice were inoculated with P. aeruginosa-laden agarose beads on day 0, and the survivors were sacrificed on day 10. Data are represented as means ± standard deviations. Log transformation of data is indicated in brackets.

^dAM, alveolar macrophage.

^ePMN, neutrophil.

^fLymph, lymphocyte.

^gWild type, wild-type littermates of Cftr-knockout mice.