Table 1.
The differentially expressed proteins between siRNA and CON group identified by MS.
| Spot No. | Protein Accession* | Protein | MW(Da) | PI | Sequence coverage (%) | Score | Ratio** | Functions |
|---|---|---|---|---|---|---|---|---|
| 662 | Q96JB5 | Isoform 2 of CDK5 regulatory subunit-associated protein 3 | 46664 | 4.56 | 5 | 71 | −1.62 | Involved in cell proliferation |
| 683 | P02545 | Isoform ADelta 10 of Lamin-A/C | 70903 | 8.55 | 32 | 188 | −1.69 | Structural molecule activity |
| 821 | A6NBZ8 | Putative uncharacterized protein ALB | 73881 | 6.33 | 26 | 139 | −2.3 | Unknown |
| 903 | 075312 | ZPR1 | 51463 | 4.66 | 24 | 394 | −1.4 | Involved in cell proliferation, signal transduction |
| 992 | P14868 | Aspartyl-tRNA synthetase, cytoplasmic | 57499 | 6.11 | 39 | 242 | 1.5 | Aminoacylase activity, aspartate-tRNA ligase activity |
| 1257 | P14780 | matrix metalloproteinase-9 | 78429 | 5.69 | 43 | 139 | −2.1 | Breakdown of extracellular matrix |
| 1304 | P62736 | Actin, aortic smooth muscle | 42381 | 5.23 | 31 | 255 | −1.56 | Regulation of cell motility |
| 1415 | P05787 | Keratin 8 | 26765 | 4.66 | 56 | 264 | −2.2 | Maintaining cellular structural integrity and also functions in signal transduction and cellular differentiation |
| 1516 | P36952 | Serpin B5 | 42530 | 5.72 | 45 | 606 | 3 | As tumor suppressor, blocks the growth, invasion, and metastatic properties of tumors |
| 1579 | P36952 | Serpin B5 | 42530 | 5.72 | 40 | 356 | 2.2 | As tumor suppressor, blocks the growth, invasion, and metastatic properties of tumors |
| 1585 | P02675 | Fibrinogen beta chain | 56577 | 8.54 | 20 | 304 | 1.8 | Component of fibrinogen, acting as a cofactor in platelet aggregation, regulate cell adhesion and spreading |
| 1596 | P07910 | Isoform 4 of Heterogeneous nuclear ribonucleoproteins C1/C2 | 27861 | 4.55 | 34 | 361 | 1.55 | Modulating the stability and the level of translation of bound mRNA molecules, binding nucleic acid |
| 1628 | P09467 | Fructose-1,6-bisphosphatase 1 | 37190 | 6.54 | 29 | 387 | 1.73 | Gluconeogenesis regulatory enzyme |
| 1774 | B4DQE1 | Annexin A8 | 44056 | 6.19 | 46 | 35 | −3.66 | Binding site for calcium and phospholipid |
| 1988 | P78417 | Glutathione S-transferase omega-1 | 27833 | 6.23 | 24 | 261 | 1.57 | Glutathione-dependent thiol transferase and dehydroascorbate reductase activities |
| 2118 | 000233 | Isoform p27-L of 26S proteasome non-ATPase regulatory subunit 9 | 24810 | 6.46 | 46 | 441 | −1.76 | Molecular chaperone |
| 2162 | 095336 | 6-phosphogluconolactonase | 27815 | 5.7 | 68 | 649 | 1.73 | Hydrolysis of 6-phosphogluconolactone to 6-phosphogluconate |
| 1039 | P78371 | T-complex protein 1 subunit beta | 57794 | 6.01 | 25 | 392 | −1.49 | Molecular chaperone |
| 1050 | P78371 | T-complex protein 1 subunit beta | 57794 | 6.01 | 23 | 211 | −1.47 | Molecular chaperone |
| 1093 | Q09028 | Retinoblastoma binding protein 4 | 46415 | 4.9 | 11 | 186 | 1.46 | Regulating chromatin metabolism |
| 1647 | P36952 | Serpin B5 | 42530 | 5.72 | 5 | 54 | 1.44 | As tumor suppressor, blocks the growth, invasion, and metastatic properties of tumors |
| 2333 | Q9H8S9 | Mps one binder kinase activator-like 1B | 16897 | 5.35 | 8 | 99 | −1.63 | Tumor suppression by restricting proliferation and promoting apoptosis |
| 1762 | Q7L5N1 | COP9 subunit 6 | 33896 | 5.65 | 15 | 201 | −1.41 | Component of the COP9 signalosome complex involved in multiple signaling pathways |
*
Accession number of the UniProt database;
**
average ratio of siRNA group/CON group.