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. 2012 Nov 1;18(11):CR667–CR673. doi: 10.12659/MSM.883545

Figure 3.

Figure 3

Dual staining of PMNs with NucView488 Caspase-3 and sulforhodamine101-annexinV after 18 hours of culture. The image shows 3 features of apoptosis: phosphatidylserine translocation (red fluorescence), active form of caspase -3 and chromatin condensation (green fluorescence) (magnification ×200). The photo was taken using Jenalumar fluorescent microscope (Carl Zeiss, Jena).