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. 2012 May 1;18(5):BR167–BR173. doi: 10.12659/MSM.882725

Figure 4.

Figure 4

The binding ability of mAb-pZNF32-8D9 to ZNF32. (A) The expression of ZNF32 mRNA in HEK293 cells, HEK293-ZNF32 cells (transfected with pEGFP-ZNF32), HEK293-pEGFP cells (transfected with pEGFP-N1), HEK293-siZNF32 cells (transfected with siRNA-ZNF32), and HEK293-siNC cells (transfected with siRNA-negative control) were analyzed using real-time PCR. (B) The ZNF32 expression levels in Jurkat, SK-MES-1, HEK293, HEK293-ZNF32, and HEK293-siZNF32 cells were detected with mAb-pZNF32-8D9 using western blotting. (C) The ZNF32 expression of HEK293 was detected by mAb-pZNF32-8D9 (1) or mAb-pZNF32-8D9 pre-incubated with peptide49–63 at dilutions of 1:50 (2) or 1:500 (3) in western blot analysis. M means the marker scaned from PVDF membrane.