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. 2012 Jan 1;18(1):BR24–BR32. doi: 10.12659/MSM.882196

Table 1.

Primers used in qRT-PCR.

Gene Annealing temperature (°C) Accession No. Product size (bp) Number gene primer
α-SMA 58 NM_031004.2 120 F: 5-AGAAGCCCAGCCAGTCGCCATCA-3
R: 5-AGCAAAGCCCGCCTTACAGAGCC-3
NF-κB 58 NM_199267.2 101 F: 5-GACCTGGAGCAAGCCATTAGCC-3
R: 5-CGGACCGCATTCAAGTCATAGTC-3
GAPDH 58 NM_017008.3 118 F: 5-AGTTCAACGGCACAGTCAAG-3
R: 5-TACTCAGCACCAGCATCACC-3