Table 1.
Gene | Annealing temperature (°C) | Accession No. | Product size (bp) | Number gene primer |
---|---|---|---|---|
α-SMA | 58 | NM_031004.2 | 120 | F: 5-AGAAGCCCAGCCAGTCGCCATCA-3 |
R: 5-AGCAAAGCCCGCCTTACAGAGCC-3 | ||||
NF-κB | 58 | NM_199267.2 | 101 | F: 5-GACCTGGAGCAAGCCATTAGCC-3 |
R: 5-CGGACCGCATTCAAGTCATAGTC-3 | ||||
GAPDH | 58 | NM_017008.3 | 118 | F: 5-AGTTCAACGGCACAGTCAAG-3 |
R: 5-TACTCAGCACCAGCATCACC-3 |