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. 2012 Aug 1;18(8):BR299–BR308. doi: 10.12659/MSM.883262

Figure 3.

Figure 3

miR-10b mimic-derived overexpression dramatically increases its level in MDA-MB-231 cells. (A) FAM-labeled miR-10b mimic was transiently transfected into MDA-MB-231 cells. The transfection efficiency was estimated 48 h later by imaging the cells under bright light (top) and fluorescence microscopy (bottom) (200×). (B) The level of miR-10b was examined by RT-qPCR at 48 h after transfection and quantified as the ratio of miR-10b to snRNA U6 (internal control), with the relative level in Mock cells arbitrarily defined as 1.0. Mock, mock-transfected cells; MI-Ctrl, control mimic-transfected cells; Blank, non-transfected cells; MI-10b, miR-10b mimic-transfected cells. * P<0.01, as compared to all other groups.