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Ginkgolic acid inhibits HIV protease activity in a concentration-dependent manner. Recombinant HIV-1 HXB2 KIIA protease and the EnzoLyte™ 520 HIV-1 protease assay kit (AnaSpec Co.) were used in this study. HIV protease inhibitor ritonavir (10.8 μg/ml or 15 μM) was used as a positive control. Ginkgolic acid (32.2, 62.6 and 125 μg/ml) and ginkgolide A (100 μg/ml) were used. ** P<0.01 and * P<0.05 as compared with the negative control (DMSO).
