Figure 2.
Expression and inhibition of PLK1 in colorectal cancer cells lines. (A) PLK1 mRNA expression detected in 9 colorectal cancer cell lines by PCR; (B) PLK1 protein expression detected in 9 colorectal cancer cell lines by Western blotting; (C) PLK1 mRNA expression detected in siRNA-treated SW1116 by real-time PCR at 24 hours after transfection. Values are mean ±SD of the expression amount of PLK1 relative to GAPDH; (D) PLK1 protein expression detected in siRNA-treated SW1116 by Western blotting at 48 hours and 72 hours after transfection.