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. 2013 Jan 2;5:ecurrents.md.3756b4a389974dff21c0cf13508d3f7b. [Version 1] doi: 10.1371/currents.md.3756b4a389974dff21c0cf13508d3f7b

PMC3560873.1; 2013 Jan 2
PMC3560873.2; 2013 Jan 2

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Deletion of asparagine 312 allows for secretion of full-length recombinant α
dystroglycan (αDG) from CHO and CHO-LARGE cells. — A plasmid encoding a full-length αDG cDNA (wild type, WT) with a FLAG epitope tag at the N-terminus, or various asparagine deletion mutants (dR), were transfected into CHO or CHO-LARGE cells and proteins purified from the supernatant using anti-FLAG agarose. Proteins were detected by immunoblotting for αDG using antibody to FLAG, αDG (IIH6 or VIA4-1) or by laminin α2 (LNα2) protein overlay.