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. 2013 Jan 2;5:ecurrents.md.3756b4a389974dff21c0cf13508d3f7b. Originally published 2013 Jan 2. [Version 2] doi: 10.1371/currents.md.3756b4a389974dff21c0cf13508d3f7b

Method for purification of delR312 αDG secreted from transfected CHO cells.

Method for purification of delR312 αDG secreted from transfected CHO cells.

CHO cells or CHO cells stably overexpressing LARGE (CHO-LARGE) were transfected with a FLAG-tagged cDNA encoding delR312 αDG. Supernatant was continuously collected and applied to anti-FLAG antibody (M2) affinity resin, which was batch eluted in 2M MgCl2. After dialysis, protein was further purified over Wheat germ agglutinin (WGA) agarose and eluted with 0.3M N-acetyl-glucosamine (GlcNAc). After dialysis, delR312 αDG protein was concentrated and studied.