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. 2013 Jan 2;5:ecurrents.md.3756b4a389974dff21c0cf13508d3f7b. Originally published 2013 Jan 2. [Version 2] doi: 10.1371/currents.md.3756b4a389974dff21c0cf13508d3f7b

Characterization of delR312 αDG purified from transfected CHO or CHO-LARGE cells.

Characterization of delR312 αDG purified from transfected CHO or CHO-LARGE cells.

FLAG-tagged delR312 αDG was purified from the supernatant of transfected CHO or CHO-LARGE cells. 5μg of post-purified protein was compared to pre-purified supernatant and post-purified, but untransfected, supernatant by silver staining and anti-FLAG immunoblotting after separation by 4-12% gradient SDS-PAGE.