Table 2. Composition of O-linked monosaccharides on delR312 αDG purified from CHO and CHO-LARGE cells.

Trimethylsialylated monosaccharides liberated from O-linked glycans were quantified by GC/MS using calibration curves with standards. Errors are SD for n=2 experiments per condition.

	CHO	CHO-LARGE	CHO-LARGE/CHO
Monosaccharide	Mole%	Mole%	Mole% Ratio
Glc	19.8±0.8	16.6±0.4	0.8±0.1
Gal	6.1±0.6	7.6±0.7	1.2±0.2
Man	1.4±0.5	2.2±0.2	1.6±0.4
Man-ol	55.6±8.6	54.7±0.1	1.0±0.1
GlcNAc	2.6±0.2	2.1±0.1	0.8±0.1
GalNAc	0.9±0.1	0.7±0.2	0.8±0.1
GalNac-ol	0.6±0.1	3.1±0.3	5.2±0.7
NeuNAc	5.1±1.3	2.0±1.1	0.4±0.2
Fuc	0.5±0.1	6.9±0.3	13.8±1.7
Xyl	0.1±0.0	4.4±0.1	43±0.5