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Osteogenic differentiation of bone marrow derived MSC. Confirmation of osteogenesis using von Kossa staining at early-P3 (a–e) and late passages-P20 (f–j) control: a, f; DMEM-LG: b, g; ALPHA-MEM: c, h; DMEM-F12: d, i; DMEM-KO: e, j) Alizarin Red staining at early-P3 (k–o) and late passages-P20 (p–t) control: k, p; DMEM-LG: l, q; ALPHA-MEM: m, r; DMEM-F12: n, s; DMEM-KO: o, t
