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23Na-NMR spectra acquired with control buffer (a) and with shift reagent (SR) TmDOTP containing buffer. In the absence of TmDOTP (control buffer, a), the on-resonance was seen at 0 ppm. After 10 min of continuous perfusion with TmDOTP, Na+e peak was separated from Na+i peak. Reference: a small glass capillary, which contained a known amount of TmDOTP
