Fig. 2.

Activated α₂M induces MCP-1 expression by Schwann cells in culture. A: Schwann cells were treated with vehicle, native α₂M (50 nM), or α₂M-MA (50 nM) for 6 hr. Levels of mRNA for MCP-1 and TNF-α were determined by qPCR (mean ± SEM). B: rα₂M, rα₂M-MA, rα₂M^1370/1374, and rα₂M^1370/1374-MA were subjected to nondenaturing PAGE. Protein was detected by Coomassie staining. C: Schwann cells were treated with vehicle, rα₂M, rα₂M-MA, rα₂M^1370/1374, or rα₂M^1370/1374-MA (each at 50 nM). MCP-1 mRNA was determined by qPCR (mean ± SEM). Expression is standardized relative to the highest value.