Fig. 4.
α2M-protease complexes induce MCP-1 expression by Schwann cells. A: α2M was reacted with increasing concentrations of active trypsin. The products were subjected to nondenaturing PAGE and Coomassie staining. The ratio (r) of active trypsin to α2M is shown. B: α2M-trypsin complex formed at the listed molar ratios was added to Schwann cell cultures for 6 hr. The total concentration of α2M (trypsin-activated and unactivated) was 50 nM. MCP-1 mRNA was determined (mean ± SEM, *P < 0.05, **P < 0.01).