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. 2012 Nov 27;161(2):1021–1033. doi: 10.1104/pp.112.209718

Figure 1.

Figure 1.

Outline of the PPHAS expression cassette for seed-specific antibody expression. PPHAS, Promoter of the β-PHASEOLIN gene from bean; 5′ UTR, 5′ untranslated region; 2S2, signal peptide of the 2S2 SSP gene from Arabidopsis; VHH-Fc, coding sequence of the VHH-Fc antibody fusion; scFv-Fc, coding sequence of the scFv-Fc antibody fusion; KDEL, ER-retention signal; 3′ARC5-I, 3′ end of the ARCELIN5-I gene. In the VHH-Fc-containing construct, the VHH-Fc coding region is flanked by attB Gateway recombination sites (De Buck et al., 2012; S. De Buck, J. Nolf, T. De Meyer, V. Virdi, K. De Wilde, E. Van Lerberge, B. Van Droogenbroeck, and A. Depicker, unpublished data). The structure is not drawn to scale.