Table II. Differentially up-regulated genes in 13-dpa developing Arabidopsis seeds shared by all three lines expressing recombinant antibody fragments.
Expression of underlined genes was validated by qPCR. References are as follows: 1, Martínez and Chrispeels (2003); 2, Kamauchi et al. (2005).
| Gene |
Descriptiona |
Name |
Fold Change |
UPR Gene |
Reference |
||
|---|---|---|---|---|---|---|---|
| HC28 |
HC26 | MBP39 | |||||
| Protein folding | |||||||
| At5g28540 | Luminal binding protein1 (HSP70 family) | BIP1 | 4.76 | 4.76 | 2.6 | x | 1, 2 |
| At5g42020 | Luminal binding protein2 (HSP70 family) | BIP2 | 5.24 | 5.81 | 2.96 | x | 2 |
| At1g09080 | Luminal binding protein3 (HSP70 family) | BIP3 | 18.19 | 16.79 | 7.27 | x | 1, 2 |
| At1g77510 | PDI-like (PDIL) protein6 (TRX superfamily) | AtPDI6 | 8.91 | 9.82 | 8.56 | x | 2 |
| At2g32920 | PDIL protein9 (TRX superfamily) | AtPDI9 | 5.66 | 6.11 | 6.48 | x | 1, 2 |
| At2g47470 | PDIL protein11 (TRX superfamily) | AtPDI11 | 4 | 4.24 | 4.35 | x | 1, 2 |
| At5g61790 | Calnexin1, involved in protein folding, functions in UPR | CNX1 | 4.48 | 4.77 | 2.22 | x | 1, 2 |
| At5g07340 | Calnexin2, involved in protein folding, functions in UPR | T2I1.50 | 6.78 | 6.45 | 3.2 | x | 1, 2 |
| At3g08970 | J domain protein, localized in ER lumen (similarity to HSP40) and is induced by heat stress | TMS1 | 6.93 | 5.99 | 7.49 | ||
| Glycosylation/modification | |||||||
| At2g02810 | UDP-Gal and UDP-Glc transporter into Golgi, localized in ER, functions in UPR | UTR1 | 4.02 | 3.97 | 2.76 | x | 1, 2 |
| Translocation/protein transport | |||||||
| At1g29310 | SecY protein transport family protein | F28N24.2 | 5.71 | 8.73 | 9.01 | x | 2 |
| At5g54860 | Major facilitator superfamily protein | MBG8.12 | 3.35 | 3.78 | 3.46 | ||
| Protein degradation | |||||||
| At4g29330 | Involved in proteolysis | DER1 | 3.09 | 2.89 | 3.42 | ||
| Vesicle trafficking | |||||||
| At3g44340 | Homologous to yeast and animal Sec24 proteins | CEF | 3.23 | 3.78 | 4.78 | ||
| Stress-related protein | |||||||
| At5g47120 | BAX inhibitor1 homolog, functions as an attenuator of biotic and abiotic types of cell death | ATBI-1 | 5.29 | 4.79 | 8.24 | x | 2 |
| TF | |||||||
| At1g25310 | Maternal effect embryo arrest8, involved in embryo development ending in seed dormancy | MEE8 | 25.34 | 6.96 | 21.58 | ||
| At1g42990 | ER stress sensor | AtBZIP60 | 4.74 | 4.33 | 6.06 | x | |
| At5g64060 | NAC domain-containing protein | ANAC103 | 4.36 | 3.14 | 12.43 | ||
| Unclassified | |||||||
| At1g18830 | Transducin/WD40 repeat-like superfamily protein | F6A14.8 | 11.21 | 18.44 | 52.38 | ||
| At4g16660 | Heat shock protein70 (Hsp70) family protein | DL4355W | 7.09 | 6.31 | 4.17 | ||
| At5g23575 | Transmembrane CLPTM1 family protein | 3.88 | 4.98 | 5.1 | |||
| At5g42050 | DCD (development and cell death) domain protein | MJC20.15 | 3.2 | 3.4 | 4.93 | ||
| At1g64460 | Protein kinase superfamily protein | F1N19.4 | 2.8 | 3.21 | 3.18 | ||
| At1g64470 | Ubiquitin-like superfamily protein | F1N19.30 | 3.66 | 3.3 | 4.34 | ||
| At3g18080 | B-S glucosidase44 | BGLU44 | 3.05 | 3.18 | 3.81 | ||
| At1g78920 | Vacuolar H+-pyrophosphatase2 protein, functions as protein pump of the Golgi apparatus | AVP2 | 2.66 | 2.97 | 3.43 | ||
| At5g64510 | Located in endomembrane system, tunicamycin induced, function and biological process unknown | MUB3.3 | 7.99 | 6.8 | 5.18 | x | b |
a
Data relating to gene function were mainly retrieved from the TAIR database.