Folate levels in leaves of OX, KD, and wild-type lines. Folates were extracted from the third leaf collected from 16-leaf-stage T1 and wild-type plants. Four OX lines, four KD lines, and four wild-type plants were analyzed. Equal amounts of leaf tissues from five T1 heterozygous plants of each line were pooled for folate analysis. The bars represent folate levels as averages ± sd of three biological replicates. FW, Fresh weight. *P < 0.05, **P < 0.01. [See online article for color version of this figure.]