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. 2012 Dec 18;161(2):628–643. doi: 10.1104/pp.112.211094

Table V. Enzymatic activities in breaker fruits in the PEPCK lines.

Values are presented as means ± se of six biological determinations. Values that are significantly different by Student’s t test from the wild type are set in boldface (P < 0.05). Vsel, Absence of dithiothreitol; Vred, presence of dithiothreitol.

Enzymatic Activities Wild Type PEPCK7 PEPCK10 PEPCK11
AGPase + 3PGA (nmol min−1 g−1 fresh wt) 15.67 ± 1.37 11.23 ±1.20 10.98 ± 0.98 13.34 ± 1.17
AGPase3PGA (nmol min−1 g−1 fresh wt) 11.37 ± 1.02 9.34 ± 0.89 8.99 ± 0.94 10.37 ± 0.85
AGPase activation state (Vsel/Vred) 0.82 ± 0.04 0.71 ± 0.03 0.73 ± 0.02 0.73 ± 0.01
NADP-MDH total (nmol min−1 g−1 fresh wt) 0.53 ± 0.07 0.37 ± 0.03 0.41 ± 0.02 0.38 ± 0.07
NADP-MDH initial (nmol min−1 g−1 fresh wt) 0.15 ± 0.04 0.14 ± 0.07 0.15 ± 0.05 0.12 ± 0.06
NADP-MDH activation stage (nmol min−1 g−1 fresh wt) 0.82 ± 0.05 0.78 ± 0.07 0.8 ± 0.03 0.75 ± 0.07
NAD-MDH (μmol min−1 g−1 fresh wt) 4.52 ± 0.32 4.33 ± 0.41 4.83 ± 0.27 4.99 ± 0.37
PK (nmol min−1 g−1 fresh wt) 0.83 ± 0.09 0.75 ± 0.07 0.88 ± 0.05 0.69 ± 0.07
PEPC (μmol min−1 g−1 fresh wt) 0.29 ± 0.06 0.26 ± 0.04 0.27 ± 0.07 0.24 ± 0.03
NAD-ME (μmol min−1 g−1 fresh wt) 0.61 ± 0.05 0.50 ± 0.04 0.59 ± 0.05 0.52 ± 0.06
NADP-ME (μmol min−1 g−1 fresh wt) 1.23 ± 0.08 0.87 ± 0.05 1.15 ± 0.09 1.26 ± 0.08