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. 2012 Dec 20;161(2):644–662. doi: 10.1104/pp.112.210997

Figure 2.

Figure 2.

Dual targeting of DNA topoisomerase to mitochondria and plastids. A, Phylogenetic analysis of genes encoding DNA topoisomerases (Top) from Arabidopsis (At), rice (Os), P. patens (Pp), P. glauca (Pg), C. reinhardtii (Cr), and C. variabilis (Cv) using MEGA 5 (see “Materials and Methods”). B, Table of Top proteins from Arabidopsis, rice, P. patens, P. glauca, C. reinhardtii, and C. variabilis with genomic loci numbers, targeting predictions (see “Materials and Methods”), and experimental localization based on GFP targeting. C, GFP images of the targeting ability of tested Top proteins. Dual targeting of AtTopIA1 and OsTopI was evident in Arabidopsis cell suspensions and onion epidermal cells. In contrast, dual targeting of PpTop was only evident in onion epidermal cells and P. patens protonemal tissues. Mitochondria (M) and plastids (Pl) are indicated, respectively. Scale bar indicates 20 µm. Cyto, Cytosol; N, nuclear; S, secretory pathway.