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. 2012 Dec 20;161(2):644–662. doi: 10.1104/pp.112.210997

Figure 3.

Figure 3.

Dual targeting of DNA helicase to mitochondria and plastids. A, Phylogenetic analysis of genes encoding DNA helicase (Hel) from Arabidopsis (At), rice (Os), P. patens (Pp), C. reinhardtii (Cr), and C. variabilis (Cv) using MEGA 5 (see “Materials and Methods”). There was no ortholog found in P. glauca. B, Table of Hel proteins from Arabidopsis, rice, P. patens, C. reinhardtii, and C. variabilis with genomic loci numbers, targeting predictions, and experimental localization based on GFP tagging. C, GFP images of the targeting ability of Hel proteins tested. Dual targeting of OsHel and PpHel1 was evident in Arabidopsis cell suspensions, onion epidermal cells, and P. patens protonemal tissues. Mitochondria (M) and plastids (Pl) are indicated, respectively. Scale bar indicates 20 µm. Cyto, Cytosol; ER, endoplasmic reticulum; N, nuclear.