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. 2012 Dec 20;161(2):644–662. doi: 10.1104/pp.112.210997

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© 2013 American Society of Plant Biologists. All Rights Reserved.

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Figure 6. — Dual targeting of APX to mitochondria and plastids. A, Phylogenetic analysis of genes encoding APX from Arabidopsis (At), rice (Os), P. glauca (Pg), P. patens (Pp), and C. reinhardtii (Cr) using MEGA 5 (see methods). There was no ortholog found in C. variabilis. B, GFP images of targeting ability of tested APX proteins. Dual targeting of AtSAPX was evident in Arabidopsis cell suspensions and onion epidermal cells. Four rice APX proteins were tested. OsAPX5 and OsAPX6 showed targeting to mitochondria, while OsAPX7 and OsAPX8 showed targeting to plastids. PgAPX1 showed targeting to both mitochondria and plastids in onion epidermal cells, while PpAPX1 showed targeting to plastids only in all tested tissues. C, Table of the APX proteins from Arabidopsis, rice, and P. patens with genomic loci numbers, targeting predictions, and experimental localization based on GFP targeting. Mitochondria (M) and plastids (Pl) are indicated, respectively. Scale bar indicates 20 µm. Cyto, Cytosol; ER, endoplasmic reticulum.

Figure 6. — Dual targeting of APX to mitochondria and plastids. A, Phylogenetic analysis of genes encoding APX from Arabidopsis (At), rice (Os), P. glauca (Pg), P. patens (Pp), and C. reinhardtii (Cr) using MEGA 5 (see methods). There was no ortholog found in C. variabilis. B, GFP images of targeting ability of tested APX proteins. Dual targeting of AtSAPX was evident in Arabidopsis cell suspensions and onion epidermal cells. Four rice APX proteins were tested. OsAPX5 and OsAPX6 showed targeting to mitochondria, while OsAPX7 and OsAPX8 showed targeting to plastids. PgAPX1 showed targeting to both mitochondria and plastids in onion epidermal cells, while PpAPX1 showed targeting to plastids only in all tested tissues. C, Table of the APX proteins from Arabidopsis, rice, and P. patens with genomic loci numbers, targeting predictions, and experimental localization based on GFP targeting. Mitochondria (M) and plastids (Pl) are indicated, respectively. Scale bar indicates 20 µm. Cyto, Cytosol; ER, endoplasmic reticulum.