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. 2012 Nov 30;161(2):880–892. doi: 10.1104/pp.112.207142

Figure 4.

Figure 4.

Expression of TaMATE1B in tobacco and rice increases constitutive citrate efflux. TaMATE1B cDNA was constitutively expressed in tobacco and rice plants using A. tumefaciens-mediated transformation. Independent T0 plants were used to generate T1 seed for measurements of citrate efflux and TaMATE1B expression in tobacco (A and B) and rice (C and D). Citrate efflux data (A and C) show means ± se (n = 3 or 5). Efflux from tobacco was measured on three replicate batches of 40 seedlings for each T1 line. Citrate efflux from rice was measured on excised root apices collected from five plants in each T1 family. TaMATE1B expression is expressed relative to the Malate Dehydrogenase gene in tobacco (B) and to GAPDH in rice (D). Expression data are means ± se from three (tobacco) or four (rice) biological replicates. Different letters indicate genotypes that are significantly different from one another (P < 0.05) as identified by one-way ANOVA.