Figure 5.

Ethylene represses in vivo Ser-62 phosphorylation of ERF110 protein. Both wild-type Arabidopsis seedlings and constitutive ethylene-response mutant ctr1-1 seedlings were grown to 2 weeks of age on M/S medium supplemented with 100 µm AOA. These two groups of plants were treated either with air (Air) or 10 µL L⁻¹ ethylene (Ethylene) for 12 h. The total cellular proteins were extracted from both groups of plants. The amount of ERF110 protein loaded onto each lane was immunoprecipitated from 10 mg of total cellular proteins. Western-blot analysis was performed using a mouse monoclonal antibody raised against the Ser-62 phosphosite of ERF110 and a rabbit polyclonal antibody raised against ERF110 in order to examine the relative abundance of both endogenous ERF110 and its Ser-62-phosphorylated isoform.