Figure 1.

PYL8 plays a nonredundant role in root sensitivity to ABA. A, Quantification of ABA-mediated root growth inhibition of pyr/pyl mutants compared with the wild type. Data are averages ± se from three independent experiments (n = 15 each). *P < 0.01 (Student’s t test) with respect to the wild type in the same experimental condition. B, Seedling establishment of pyr/pyl mutants compared with Col and Landsberg erecta (La-er) wild types in medium supplemented with 0.5 μm ABA at 3, 5, 7, and 10 d after sowing. Data show the percentage of seeds that germinated and developed green cotyledons. Values are averages ± se for three independent experiments (100 seeds each). *P < 0.01 (Student’s t test) with respect to the wild type in the same experimental condition. C, ABA-insensitive phenotypes of pyl8-1 and pyl8-2 alleles compared with the Col wild type. Photographs show representative seedlings 10 d after the transfer of 4-d-old seedlings from Murashige and Skoog medium (MS) to plates lacking or supplemented with 10 μm ABA. D, Complementation of the pyl8-1 allele by introduction of a 35S:3HA-PYL8 transgene (pyl8-1 complemented). The photograph shows representative seedlings 12 d after the transfer of 4-d-old seedlings from Murashige and Skoog medium to plates supplemented with 20 μm ABA. [See online article for color version of this figure.]