Table I. Summary of the coprecipitation of Cys-substituted Tha4 by Cys-substituted pOE17.
Coprecipitations were conducted as described in “Materials and Methods” and as represented in Figure 2 for pOE17K99C. Results are averages of at least three separate experiments. Coprecipitation is indicated by +, no coprecipitation is indicated by −, and weak coprecipitation is indicated by +/−. n.d., Not determined.
| Precursor |
||||
|---|---|---|---|---|
| Tha4 Residue | pOE17D59C | pOE17D68C | pOE17S84C | pOE17T115C |
| N terminus | ||||
| G5C | n.d. | + | n.d. | + |
| L6C | n.d. | + | n.d. | n.d. |
| P9C | + | + | +/− | − |
| Transmembrane | ||||
| G16C | − | n.d. | − | − |
| APH | ||||
| V30C | + | n.d. | n.d. | n.d. |
| V38C | + | n.d. | + | n.d. |
| K39C | n.d. | + | n.d. | n.d. |
| S40C | n.d. | + | n.d. | n.d. |
| F41C | n.d. | + | n.d. | n.d. |
| K46C | +/− | + | +/− | +/− |
| E47C | n.d. | + | +/− | n.d. |
| F48C | + | + | n.d. | + |
| T50C | + | + | n.d. | + |
| K53C | + | + | + | + |
| C-tail | ||||
| A65C | − | n.d. | +/− | − |