Figure 8. Autophagy deficiency results in an increased frequency of aneuploidy after the addition of a nitrogen source.

ATG2 (DBY4962) and Δatg2 (AMY262) cells arrested at G1 by α-factor were released into SC-Ura medium. Synchronous cultures were collected after 0.75 h and re-released into SD-N medium. After 25 h and 49 h, cell cultures were re-released into SCD medium. Appropriately diluted 0.75, 25+2 and 49+2 h aliquots were plated onto YEPD plates and selective medium lacking both leucine and uracil. Colonies were scored after 4 days at 30°C.