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. 2013 Jan 31;9(1):e1003244. doi: 10.1371/journal.pgen.1003244

Figure 6. Expression patterns of PIF-encoding genes.

Figure 6

(A–E) Representative images of histochemical staining of GUS activity in 2-d-old dark-grown transgenic seedlings. The GUS reporter gene is driven by PIF1 (A), PIF3 (B), PIF4 (C), PIF5 (D) and CaMV 35S (E) promoters, respectively. Data of biological triplicates were collected from two independent transgenic lines, and representative images are shown for each transgene. (F) Steady-state transcript levels of PIF-encoding genes defined by RNA-seq and RT-qPCR analyses in 2-d-old dark-grown WT seedlings. Data are presented as the mean of biological triplicates +/− SEM. (G) Relative expression of PIF-encoding genes in 2-d-old dark-grown pifq and pif-triple mutants. Expression was determined by RT-qPCR, normalized to an internal PP2AA3 control, and presented relative to WT levels set at unity. Data are represented as the mean of biological triplicates +/− SEM.