Table 1.

Primer sequences

Name	Primer Sequences (5’ – 3’)	Product Size Conventional Or real-time	Target Gene(GenBank Accession)
β2-microglobulin	F: CTTATGCACGCTTAACTATCTTAACAA R: TAGGAGGGCTGGCAACTTAG	127bp Real-time	B2M NM_004048
Calponin	F: GATGGCATCATTCTTTGCGA R: TTGTAGTAGTTGTGTGCGTG F: CTGAGAGAGTGGATCGAGGG R: TGATCTTCTTCACGGAGCCT	701bp Conventional 127bp Real-time	CNN1 NM_001299
Connexin 43	F: GTGCCTGAACTTGCCTTTTC R: GATGATGTAGGTTCGCAGCA F: GGAGTTCAATCACTTGGCGT R: ACACCTTCCCTCCAGCAGTT	522bp Conventional 134bp Real-time	GJA1 NM_000165
Cyclo-oxygenase-type 2 also known as Prostaglandin GH synthase-2	F: AGATCATCTCTGCCTGAGTATCTT R: TTCAAATGAGATTGTGGAAAAATTGCT F: GCTGGGCCATGGGGTGGACT R: CCTGCCCCACAGCAAACCGT	305bp Conventional 200bp Real-time	COX-2 or PTGS-2 NM_000963
Glyceraldehyde-phosphatedehydroegnase	F: CCACCCATGGCAAATTCCATGGCA R: TCTAGACGGCAGGTCAGGTCCACC	598bp Conventional	GAPDH NM_002046
Vimentin	F: TCAGAGAGAGGAAGCCGAAA R: GTGAGGGACTGCACCTGTCT F: GGCTCAGATTCAGGAACAGCATG R: CCTGTCTCCGGTACTCAGTGGAC	380bp Conventional 230bp Real-time	VIM NM_003380

Sequence of primers used for conventional and real-time RT-PCR analysis.