Table 1.
Results based on plate wash of cultures from the four groups of samples, presented on the different test days with Ct-values and observation of Dichelobacter nodosus-suspect colonies
| |
Soil 5°C |
|
Soil and hoof powder 5°C |
Soil 15°C |
Soil and hoof powder 15°C |
|||
|---|---|---|---|---|---|---|---|---|
| Day | Ct-valuea | Coloniesb | Ct-value | Colonies | Ct-value | Colonies | Ct-value | Colonies |
| 4 |
17.19 |
Y |
17.53 |
Y |
18.83 |
N |
18.47 |
Y |
| |
17.28 |
Y |
18.15 |
Y |
17.12 |
Y |
15.52 |
Y |
| 7 |
18.08 |
Y |
18.52 |
Y |
34.02 |
Y |
22.68 |
N |
| |
18.64 |
N |
16.91 |
Y |
34.08 |
N |
22.02 |
N |
| 11 |
21.60 |
N |
19.42 |
Y |
33.34 |
N |
31.15 |
N |
| |
25.15 |
N |
18.24 |
Y |
33.97 |
N |
32.13 |
N |
| 14 |
24.04 |
Y |
16.51 |
Y |
35.87 |
N |
31.77 |
N |
| |
22.50 |
N |
18.95 |
Y |
30.87 |
N |
45 |
N |
| 17 |
29.95 |
N |
19.98 |
Y |
45 |
N |
29.88 |
N |
| |
25.10 |
Y |
17.31 |
Y |
45 |
N |
30.64 |
N |
| |
25.45 |
N |
18.16 |
N |
45 |
N |
29.26 |
N |
| 21 |
45 |
Y |
24.92 |
Y |
45 |
N |
32.39 |
N |
| |
45 |
Y |
34.51 |
Y |
45 |
N |
31.65 |
N |
| |
26.41 |
N |
45 |
Y |
45 |
N |
33.07 |
N |
| 24 |
36.57 |
Y |
32.25 |
N |
45 |
N |
36.11 |
N |
| |
34.23 |
N |
19.27 |
N |
45 |
N |
31.87 |
N |
| |
35.19 |
N |
25.45 |
Y |
45 |
N |
31.56 |
N |
| 28 |
45 |
N |
36.07 |
N |
45 |
N |
31.63 |
N |
| |
34.83 |
N |
31.26 |
N |
45 |
N |
30.71 |
N |
| 45 | N | 32.57 | N | 45 | N | 33.00 | N | |
a: All Ct-values presented are for the 1:10 diluted samples. On day 4–14 samples were analyzed in duplicates whereas triplicates were used from day 17 to 28.
b: Observed D. nodosus-suspect colonies on TASH agar plates (Yes/No).