Table 1.

pGOv5-based constructs^a

Construct	Insert	Intron 1e	Intron 2
pNH001	[(G4S)3]::C-TAP-tag::2xNLS b	-	-
pNH002	N-TAP-tag::[(G4S)3]::mTFP1 c	-	-
pNH009	N-TAP-tag::mTFP1	-	-
pNH013	mTFP1 d	-	-
pNH026	mTFP1::[(G4S)3]::C-TAP-tag	-	-
pNH030	mTFP1::[(G4S)3]::2xNLS	-	-
pNH078	mTFP1[1I] d	B	-
pNH082	mTFP1[2I] d	B	C

^a All constructs are in E. coli DH5α and are available from Addgene ^b lacks intact open-reading frame; ^c mTFP1 sequence lacks N-terminal Met-Val and C-terminal Lys residues present in native sequence; ^d N-terminal Met-Val residues and C-terminal Lys residue, present in native coding sequence, replaced, respectively, with Met-Ala-Ala and Val-Ser-Ala; ^e see Additional file 1: Table S2.