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. 2013 Jan 31;8(1):e55513. doi: 10.1371/journal.pone.0055513

Figure 1. Transient over-expression of miR-148a in PDAC cell lines.

Figure 1

(A) Capan-2, PANC-1, MIA PaCa-2 and BxPC-3 PDAC-derived cell lines and non-cancerous HPNE cell line were transiently transfected with miR-CT or miR-148a precursors. Four days after transfection, cell viability was assessed by colorimetric methods. For each cell line, the miR-148a-cell viability was compared to miR-CT cell viability (100%). The results are the mean of three independent experiments (±SEM) and are expressed as percentage of cell viability of control cells. (B), Cell cycle distribution was measured by propidium iodide staining followed by FACS analysis 72 hours after transfection of Capan-2 cells with miR-CT or miR-148a precursors.