Table 1. Primers used for gene expression analysis in the study.
Primer | Sequence (5′– 3′) | Tm (°C) | Products Length (bp) |
C1F | GGTGTGAACGCCATGCTGAG | 62°C | 323 bp |
C1R | GTGCACACCACATCATCGGC | ||
C2F | ACCTCCACCACTGTGCGAAC | 58°C | 293 bp |
C2R | CGGAAGTCGTCTCAGGCAGT | ||
R1F | AGAGATCGTGCAGGATGGCG | 58°C | 383 bp |
R1R | GGTGGGTATGCGTTTCGGTC | ||
R2F | GGCCACCATCATGACGAAGG | 62°C | 342 bp |
R2R | ACCCACTGCTTCCAGCCACG | ||
R4F | TCCGTCTTCTGAGCAACTGG | 62°C | 351 bp |
R4R | GCCTGCTTTGACAGTAACCA | ||
R7F | TCTTCTCAGCAGCGACAACT | 62°C | 377 bp |
R7R | ATCAGGCTCTCTGGAAGGTT | ||
C2-GF | ACTCAGCGTCCAGTGTTCTAG | 61°C | 512 bp |
C2-GR | CGGAAGTCGTCTCAGGCAGT | ||
C2-AI-F | TCGCCACCATGCCTAACTTTTCTGGCAAC | 55°C | 460 bp |
C2-AI-R | CTCTCGGACGTAGACCCTG | ||
MyoD-GF | GACAGGGAGGAGGGGTAGAG | 61.4°C | 1166 bp |
MyoD-GR | GAAGAACCAGGGACACCATC | ||
GAPDH-F | GGTGAAGGTCGGTGTGAACG | 60°C | 233 bp |
GAPDH-R | CTCGCTCCTGGAAGATGGTG |
C1F, C1R; C2F, C2R; R1F, R1R; R2F, R2R; R4F, R4R; R7F, R7R: primers used for determining the expression of CRABP1, CRABP2, CRBP1, CRBP2, RBP4, RBP7 during C2C12 differentiation time; C2-GF, C2-GR and C2-AI-F, C2-AI-R: primers used for construction of the CRABP2 over-expression vector; MyoD-GF, MyoD-GR: primers used for construction of the MyoD over-expression vector; GAPDH-F, GAPDH-R: primers used as an internal control.