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. 2013 Jan 31;8(1):e55479. doi: 10.1371/journal.pone.0055479

Table 1. Primers used for gene expression analysis in the study.

Primer Sequence (5′– 3′) Tm (°C) Products Length (bp)
C1F GGTGTGAACGCCATGCTGAG 62°C 323 bp
C1R GTGCACACCACATCATCGGC
C2F ACCTCCACCACTGTGCGAAC 58°C 293 bp
C2R CGGAAGTCGTCTCAGGCAGT
R1F AGAGATCGTGCAGGATGGCG 58°C 383 bp
R1R GGTGGGTATGCGTTTCGGTC
R2F GGCCACCATCATGACGAAGG 62°C 342 bp
R2R ACCCACTGCTTCCAGCCACG
R4F TCCGTCTTCTGAGCAACTGG 62°C 351 bp
R4R GCCTGCTTTGACAGTAACCA
R7F TCTTCTCAGCAGCGACAACT 62°C 377 bp
R7R ATCAGGCTCTCTGGAAGGTT
C2-GF ACTCAGCGTCCAGTGTTCTAG 61°C 512 bp
C2-GR CGGAAGTCGTCTCAGGCAGT
C2-AI-F TCGCCACCATGCCTAACTTTTCTGGCAAC 55°C 460 bp
C2-AI-R CTCTCGGACGTAGACCCTG
MyoD-GF GACAGGGAGGAGGGGTAGAG 61.4°C 1166 bp
MyoD-GR GAAGAACCAGGGACACCATC
GAPDH-F GGTGAAGGTCGGTGTGAACG 60°C 233 bp
GAPDH-R CTCGCTCCTGGAAGATGGTG

C1F, C1R; C2F, C2R; R1F, R1R; R2F, R2R; R4F, R4R; R7F, R7R: primers used for determining the expression of CRABP1, CRABP2, CRBP1, CRBP2, RBP4, RBP7 during C2C12 differentiation time; C2-GF, C2-GR and C2-AI-F, C2-AI-R: primers used for construction of the CRABP2 over-expression vector; MyoD-GF, MyoD-GR: primers used for construction of the MyoD over-expression vector; GAPDH-F, GAPDH-R: primers used as an internal control.