Table 2.
Summary of end-product yields, optimal growth temperatures, total molar reduction values of H2 + ethanol (RVEP), and growth conditions employed
Organism |
Growth temp (°C) |
End products (mol/mol hexose equivalent) |
|
Growth condition |
Ref |
|||||
---|---|---|---|---|---|---|---|---|---|---|
H2 | CO2 | Acetate | Ethanol | Formate | Lactate | RVEP | ||||
Ca. saccharolyticus DSM 8903 |
70 |
4.0 |
1.8 |
NR |
ND |
ND |
ND |
4.0 |
Cont., 1.1 g l-1 glucose (D = 0.09 h-1) |
[24] |
|
|
3.6 |
1.5 |
1.6 |
ND |
ND |
ND |
3.6 |
Cont., 4.1 g l-1 glucose (D = 0.1 h-1) |
[24] |
|
|
3.5 |
NR |
2.1 |
NR |
NR |
NR |
3.5 |
Batch, 10 g l-1 sucrose |
[25] |
|
|
2.5 |
1.4 |
1.4 |
ND |
ND |
0.1 |
2.5 |
Batch, 10 g l-1 glucose |
[26] |
Ca. bescii DSM 6725 |
75 |
✓ |
✓ |
✓ |
NR |
NR |
✓ |
NA |
|
[27,28] |
P. furiosus DSM 3638 |
90 |
3.8 |
1.9 |
1.5 |
0.1 |
NR |
NR |
4.0 |
Cont, cellobiose (D = 0.45 h-1) |
[29]A |
|
|
3.5 |
1.0 |
1.4 |
ND |
NR |
ND |
3.5 |
Batch, 1.9 g l-1, maltose |
[30]A |
|
|
2.9 |
1.9 |
0.8 |
0.1 |
NR |
ND |
3.1 |
Batch, 2 g l-1 maltose |
[31]B |
|
|
2.8 |
0.9 |
1.2 |
ND |
NR |
ND |
2.8 |
Batch, 3.5 g l-1, cellobiose |
[30]A |
|
|
2.6 |
1.4 |
1.0 |
ND |
NR |
NR |
2.6 |
Cont, maltose (D = 0.45 h-1) |
[29]A |
Th. kodakaraensis KOD1 |
85 |
3.3 |
1.8 |
1.1 |
NR |
NR |
NR |
3.3 |
Cont, starch (D = 0.2 h-1) |
[32]C |
T. neapolitana DSM 4359 |
80-85 |
3.8 |
2.0 |
1.8 |
ND |
NR |
0.1 |
3.8 |
Batch, 2.5 g l-1 glucose |
[33] |
|
|
3.2 |
NR |
1.9 |
NR |
NR |
NR |
3.2 |
Batch (N2 sparged), 7.0 g l-1 glucose |
[34] |
|
|
2.4 |
NR |
1.1 |
NR |
NR |
0.7 |
2.4 |
Batch, 1.1 g l-1 glucose |
[35] |
|
|
1.8 |
NR |
1.0 |
NR |
NR |
NR |
1.8 |
Batch, 7.5 g l-1 glucose |
[40] |
|
|
1.8 |
NR |
1.5 |
NR |
NR |
NR |
1.8 |
Batch, 7.0 g l-1 glucose |
[34] |
T. petrophila RKU-1 |
80 |
3.7 |
0.4 |
1.8 |
NR |
NR |
0.3 |
3.7 |
Batch, 1 g l-1 glucose |
[36] |
T. maritima MSB8 |
80 |
4.0 |
2.0 |
2.0 |
NR |
ND |
NR |
4.0 |
Batch, 2 g l-1 glucose |
[38] |
|
|
2.2 |
1.1 |
1.0 |
ND |
NR |
0.3 |
2.2 |
Batch, 3 g l-1 glucose |
[39] |
|
|
1.7 |
NR |
1.0 |
NR |
NR |
NR |
1.7 |
Batch, 7.5 g l-1 glucose |
[40] |
Cal. subterraneus subsp. tengcongensis MB4 |
75 |
2.8 |
NR |
1.4 |
0.6 |
NR |
ND |
4.0 |
Cont, starch (D = 0.27 h-1) |
[42] |
|
|
NR |
NR |
2.0 |
ND |
NR |
ND |
NA |
Cont (N2 sparged), glucose (D = 0.24 h-1) |
[42] |
|
|
0.3 |
1.5 |
1.0 |
0.7 |
NR |
ND |
1.7 |
Batch, 4 g l-1 glucose |
[41] |
E. harbinense YUAN-3 T |
35 |
2.8 |
✓ |
0.7 |
1.1 |
ND |
ND |
5.0 |
Batch, 20 g l-1 glucose |
[43] |
C. cellulolyticum H10 |
37 |
1.6 |
1.0 |
0.8 |
0.3 |
ND |
NR |
2.2 |
Batch, 5 g l-1 cellulose |
[44] |
|
|
1.8 |
1.1 |
0.8 |
0.4 |
ND |
NR |
2,6 |
Batch, 5 g l-1 cellobiose |
[44] |
C. phytofermentans ISDg |
35-37 |
Major |
Major |
0.6 |
1.4 |
0.1 |
0.3 |
NA |
Batch, 34 g l-1 cellobiose |
[45] |
|
|
1.0 |
0.9 |
0.6 |
0.5 |
0.1 |
NR |
2.0 |
Batch, 5 g l-1 cellulose |
[44] |
|
|
1.6 |
1.2 |
0.6 |
0.6 |
ND |
NR |
2.8 |
Batch, 5 g l-1 cellobiose |
[44] |
C. thermocellum ATCC 27405 |
60 |
0.8 |
1.1 |
0.7 |
0.8 |
0.3 |
ND |
2.4 |
Batch, 1.1 g l-1 cellobiose |
[10] |
|
|
1.0 |
0.8 |
0.8 |
0.6 |
0.4 |
0.4 |
2.2 |
Batch, 4.5 g l-1 cellobiose |
[46] |
C. thermocellum DSM 4150 |
60 |
1.8 |
1.7 |
0.9 |
0.8 |
ND |
0.1 |
3.4 |
Batch, 2 g l-1 glucose |
[47] |
|
|
0.6 |
1.8 |
0.3 |
1.4 |
ND |
0.2 |
3.4 |
Batch, 27 g l-1 cellobiose |
[47] |
Ta. pseudethanolicus 39E |
65 |
0.1 |
2.0 |
0.1 |
1.8 |
NR |
0.1 |
3.7 |
Batch, 8 g l-1 glucose |
[50] |
|
|
NR |
NR |
NR |
1.6 |
NR |
<0.1 |
3.2 |
1 g l-1 xylose |
[48] |
|
|
NR |
NR |
0.4 |
1.0 |
NR |
<0.1 |
2.0 |
Batch, 20 g l-1 xylose |
[49] |
|
|
NR |
NR |
0.2 |
0.4 |
NR |
1.1 |
0.8 |
Batch, 20 g l-1 glucose |
[49] |
G. thermoglucosidasius M10EXGD |
60 |
NR |
NR |
0.6 |
0.4 |
1.0 |
0.9 |
0.8 |
Batch, 10 g l-1 glucose |
[52] |
B cereus ATCC 14579 | 35 | NR | 0.1 | 0.2 | 0.2 | 0.3 | 1.1 | 0.4 | Batch, 3.6 g l-1 glucose | [51] |
A ~ 0.5 mol alanine per mol-hexose produced on cellobiose and maltose.
BProduces H2, CO2, volatile fatty acids, and NH3 on peptides in the absence of carbon source.
C ~ 0.5 mol alanine per mol-hexose produced on starch.
DOnly G. thermoglucosidasuis strain C56-TS93 has been sequenced but no end-product data is available. Strain M10EXG was used for end-product yield comparisons instead.
Abbreviations: NR, not reported; ND, not detected; NA, not applicable; Major, reported as major product without absolute values; ✓, reported as present with no values indicated; Cont, continuous culture; D, dilution rate.